PRODUCTS
BreakMapâ„¢
A rapid genome-wide on and off-target analysis platform in your own hands, powered by INDUCE-seq®
Run µþ°ù±ð²¹°ì²Ñ²¹±èâ„¢ in-house with secured cloud-based analysis
Cell editing &
immobilization
Edited cells are prepared and immobilized in your own laboratory
DNA break labelling
& library prep
µþ°ù±ð²¹°ì²Ñ²¹±èâ„¢ reagents are used to label DNA breaks in situ and NGS libraries prepared using PCR-free workflow
Sequencing
Libraries are sequenced on a standard NGS platform
Data analysis
Secure bioinformatics platform translates raw sequencing data into ranked, actionable insights
Reporting
Receive a clear ranked list of on- and off-target edits
What's included with BreakMapâ„¢
Each µþ°ù±ð²¹°ì²Ñ²¹±èâ„¢ kit is supplied with the reagents, consumables and access required to prepare next-generation sequencing libraries from edited cells, designed to integrate seamlessly with standard equipment commonly found in gene editing laboratories
Comprehensive onboarding, including: user guides, videos and in-person training (where applicable)
Molecular biology reagents and buffers
Access to the µþ°ù±ð²¹°ì²Ñ²¹±èâ„¢ cloud-based analysis
DNA purification beads for NGS library preparation
We offer support to a broad range of use cases including:
Off-target assessment
On-target mechanism
Kinetic analysis of nuclease
Guide purity screening
Optimization of editing strategy
Data analysis & reporting:
µþ°ù±ð²¹°ì²Ñ²¹±èâ„¢ includes access to our cloud-based bioinformatics platform; results are delivered in a comprehensive report with supporting data visualizations.
Designed to convert genome-wide sequencing data into clear, actionable outputs including:
• Ranked list of off-target sites
• Genome-wide, single nucleotide resolution detection of DNA breaks
• Simultaneous assessment of on-and off-target editing events
• Relative frequency compared to control samples
• Homology to the targeted editing site
• Data suitable for discovery optimization and downstream regulatory use
Technical specifications
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• Cell-based assay
• 96-well plate format
• Kit configuration: 24 samples
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• 2 day wet lab workflow
• Sample to insights within one week
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Double-strand break generating editors including:
• Cas9• Cas12a
• Novel Cas nucleases
• TALENS
Additional systems supported, please contact to discuss specific editors -
• Both adherent and suspension cells
• Primary, Stem and Immortalized cells from diverse tissues
• Therapeutically relevant cells including T Cells, Hepatocytes, HSPCs, and iPSCs.
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• Genome-wide with single nucleotide resolution mapping of break ends
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• 4200 TapeStation system or equivalent system
• R230 Focused-ultrasonicator (and rack)
• Qubit Flex or equivalent fluorometers
• NextSeq 550 or 2000 Sequencing System
